Mouse C3a (Complement Component 3a) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-M0337-24, E-EL-M0337-48, E-EL-M0337-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: C3a

UNIProt ID: P01027

Target Synonyms: C3a-desArg; ASP;

Research Areas: Cancer, Immunology, Signal transduction

Target Species: Mouse

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse C3a concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.1 ng/mL

Detection Range: 0.16-10 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C3a. You can calculate the concentration of Mouse C3a in the samples by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Mouse C3a in samples. No significant cross-reactivity or interference between Mouse C3a and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag