Phospho-NMDAR1-S896/S897 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79465-50, BS79465-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q05586

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-NMDAR1-S896/S897 antibody at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 30 minutes and PMA at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.

Specificity: Phosphorylated

Alternative Name: GRIN1; GluN1; MRD8; NMD-R1; NMDA1; NMDAR1; NR1; NMDA 1

Immunogen: A synthetic phosphorylated peptide around S896 & S897 of human NMDAR1(NP_015566.1).

Conjugate: Unconjugated

Modification: Phosphorylated